Panamerican Journal of Trauma, Critical Care & Emergency Surgery

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VOLUME 9 , ISSUE 2 ( May-August, 2020 ) > List of Articles

RESEARCH ARTICLE

A Cycle of Freezing and Thawing as a Modified Method for Activating Platelets in Platelet-rich Plasma to Use in Regenerative Medicine

İbrahim Eker, Soner Yilmaz, Rıza A Çetinkaya, Aytekin Ünlü, Aysel Pekel, Rahşan I Sağkan, Zerrin Ertaş, Orhan Gürsel, Hacı U Muşabak, Ertuğrul Yazici, Sebahattin Yilmaz, Cengizhan Açikel, İsmail Y Avci, Ferit Avcu, Emin Kürekçi, Patrizio Petrone

Citation Information : Eker İ, Yilmaz S, Çetinkaya RA, Ünlü A, Pekel A, Sağkan RI, Ertaş Z, Gürsel O, Muşabak HU, Yazici E, Yilmaz S, Açikel C, Avci İY, Avcu F, Kürekçi E, Petrone P. A Cycle of Freezing and Thawing as a Modified Method for Activating Platelets in Platelet-rich Plasma to Use in Regenerative Medicine. Panam J Trauma Crit Care Emerg Surg 2020; 9 (2):101-104.

DOI: 10.5005/jp-journals-10030-1285

License: CC BY-NC 4.0

Published Online: 03-09-2020

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Background: Platelet-rich plasma (PRP) has been widely used to improve wound healing and tissue repair. The objective of this study was to assess a cycle of freezing/thawing and its effect in the amount of growth factors in PRP. Materials and methods: After the preparation of PRP using standard methods, the samples were equally divided into two groups: The first group was activated by adding 10% calcium gluconate, while the second group was cryopreserved at −80°C for 24 hours. Enzyme-linked immunosorbent assay was used for aliquots of PRP to measure concentrations of insulin-like growth factor-1, platelet-derived growth factor, and basic fibroblast growth factor. Results: 15 mL of whole blood was obtained from 20 volunteers and collected into citrated tubes. The mean platelet count of the donors and the autologous PRP were 238.5 ± 44.7 × 103/μL and 544.7 ± 161.5 × 103/μL, respectively. There were no significant differences between the growth factor levels of freeze-thawed and calcium-activated PRP. A cycle of freezing/thawing was the only independent factor associated with the growth factor yield in the multivariate model. Conclusion: With its features of being simple, inexpensive, and easy for standardization, a cycle of freezing/thawing may be the method of choice for PRP activation procedure, without inducing fibrin matrix. This study was performed in a laboratory setting; therefore, future clinical trials are recommended.


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